Catalysts are substances that increase the reaction rate of a chemical reaction without being consumed in the process. 707-07-3, Name is (Trimethoxymethyl)benzene, SMILES is COC(OC)(C1=CC=CC=C1)OC, belongs to ethers-buliding-blocks compound. In a document, author is Dong, Hongjing, introduce the new discover, Recommanded Product: (Trimethoxymethyl)benzene.
Purification of hypocrellins from Shiraia bambusicola by coordinated high-speed countercurrent chromatography using cupric chloride as a complexing agent
Hypocrellins are anthraquinone that can act as excellent photosensitizers for photodynamic therapy. In the present work, we found that high-speed countercurrent chromatography using cupric chloride as a complexing agent effectively separated hypocrellins from Shiraia bambusicola extract. The optimal two-phase solvent system consisted of petroleum ether/ethyl acetate/methanol/water (7:3:5.5:4.5, v/v/v/v), with 0.01 mol/L cupric chloride in the lower phase at pH of 2.45. This lower phase served as the mobile phase, whereas the upper phase acted as the stationary phase. Employing a continuous separation mode, three continuous injections were found to allow the purification of 1.2 g of crude extract in approximately 12 h. Hypocrellin B (10.8 mg), hypocrellin A (16.2 mg), and hypocrellin C (15.6 mg) were obtained from this process. Simulation of complexation of hypocrellin A with divalent copper ion by computational chemistry calculations indicated that three pairs of hydroxyl and carbonyl groups in hypocrellin A had similar binding energies, and demonstrated that hypocrellin A and B owned different metal-to-ligand ratios as compared to hypocrellin C. These factors could modify the partitioning of these compounds in two-phase solvent system, and resulting in a suitable separation factor. This method would also be used to purify other anthraquinones from natural products.
The proportionality constant is the rate constant for the particular unimolecular reaction. the reaction rate is directly proportional to the concentration of the reactant. I hope my blog about 707-07-3 is helpful to your research. Recommanded Product: (Trimethoxymethyl)benzene.