LC-QTOF-MS identification of major urinary cyclopropylfentanyl metabolites using synthesized standards was written by Vikingsson, Svante;Rautio, Tobias;Wallgren, Jakob;Strand, Anna;Watanabe, Shimpei;Dahlen, Johan;Wohlfarth, Ariane;Konradsson, Peter;Wu, Xiongyu;Kronstrand, Robert;Green, Henrik. And the article was included in Journal of Analytical Toxicology in 2019.HPLC of Formula: 2380-78-1 The following contents are mentioned in the article:
The aim of this studywas to provide the exact structure of abundant and unique metabolites of cyclopropylfentanyl along with synthesis routes. In this study, metabolites were identified in 13 post-mortem urine samples using liquid chromatog- raphy quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). Samples were analyzed with and without enzymic hydrolysis, and seven potential metabolites were synthesized inhouse to provide the identity of major metabolites. Cyclopropylfentanyl was detected in all samples, and the most abundant metabolite was norcyclo- propylfentanyl (M1) that was detected in 12 out of 13 samples. Reference materials were synthesized (synthesis routes provided) to identify the exact structure of the major metabolites 4-hydroxyphenethyl cyclopropylfentanyl (M8), 3,4-dihydroxyphenethyl cyclopropylfentanyl (M5) and 4-hydroxy-3-methoxyphenethyl cyclopropylfentanyl (M9). These metabolites are suitable urinary markers of cyclopropylfentanyl intake as they are unique and detected in a majority of hydrolyzed urine samples. Minor metabolites included two quinone metabolites (M6 and M7), not previously reported for fentanyl analogs. Interestingly, with the exception of norcyclopropylfentanyl (M1), the metabolites appeared to be between 40% and 90% conjugated in urine. In total, 11 metabolites of cyclo- propylfentanyl were identified, including most metabolites previously reported after hepatocyte incubation. This study involved multiple reactions and reactants, such as 4-Hydroxy-3-methoxyphenethanol (cas: 2380-78-1HPLC of Formula: 2380-78-1).
4-Hydroxy-3-methoxyphenethanol (cas: 2380-78-1) belongs to ethers. Esters are also usually derived from carboxylic acids. It may also be obtained by reaction of acid anhydride or acid halides with alcohols or by the reaction of salts of carboxylic acids with alkyl halides. Liquid esters of low volatility serve as softening agents for resins and plastics. Esters also include many industrially important polymers. Polymethyl methacrylate is a glass substitute sold under the names Lucite and Plexiglas; polyethylene terephthalate is used as a film (Mylar) and as textile fibres sold as Terylene, Fortrel, and Dacron.HPLC of Formula: 2380-78-1
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem