A minimal hybridization chain reaction (HCR) system using peptide nucleic acids was written by Kim, Ki Tae;Angerani, Simona;Winssinger, Nicolas. And the article was included in Chemical Science in 2021.Reference of 111-77-3 This article mentions the following:
The HCR represents a powerful tool for amplification in DNA-based circuitry and sensing applications, yet requires the use of long DNA sequences to grant hairpin metastability. Here we describe a minimal HCR system based on peptide nucleic acids (PNAs). A system comprising a 5-mer stem and 5-mer loop/toehold hairpins was found to be suitable to achieve rapid amplification. These hairpins were shown to yield > 10-fold amplification in 2 h and be suitable for the detection of a cancer biomarker on live cells. The use of γgamma-peg-modified PNA was found to be beneficial. In the experiment, the researchers used many compounds, for example, 2-(2-Methoxyethoxy)ethanol (cas: 111-77-3Reference of 111-77-3).
2-(2-Methoxyethoxy)ethanol (cas: 111-77-3) belongs to ethers. The oxygen atom in ethers are more electronegative than carbon, thus the hydrogens which are alpha to the ethers are more acidic than the simple hydrocarbons. At room temperature, ethers are pleasant-smelling colourless liquids. Relative to alcohols, ethers are generally less dense, are less soluble in water, and have lower boiling points. They are relatively unreactive, and as a result they are useful as solvents for fats, oils, waxes, perfumes, resins, dyes, gums, and hydrocarbons. Vapours of certain ethers are used as insecticides, miticides, and fumigants for soil.Reference of 111-77-3
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem