On June 18, 2021, Knorrscheidt, Anja; Soler, Jordi; Huenecke, Nicole; Puellmann, Pascal; Garcia-Borras, Marc; Weissenborn, Martin J. published an article.Quality Control of 2-Methoxynaphthalene The title of the article was Accessing chemo- and regioselective benzylic and aromatic oxidations by protein engineering of an unspecific peroxygenase. And the article contained the following:
Unspecific peroxygenases (UPOs) enable oxyfunctionalizations of a broad substrate range with unparalleled activities. Tailoring these enzymes for chemo- and regioselective transformations represents a grand challenge due to the difficulties in their heterologous productions. Herein, we performed protein engineering in Saccharomyces cerevisiae using the MthUPO from Myceliophthora thermophila. More than 5300 transformants were screened. This protein engineering led to a significant reshaping of the active site as elucidated by computational modeling. The reshaping was responsible for the increased oxyfunctionalization activity, with improved kcat/Km values of up to 16.5-fold for the model substrate 5-nitro-1,3-benzodioxole. Moreover, variants were identified with high chemo- and regioselectivities in the oxyfunctionalization of aromatic and benzylic carbons, resp. The benzylic hydroxylation was demonstrated to perform with enantioselectivities of up to 95% ee. The proposed evolutionary protocol and rationalization of the enhanced activities and selectivities acquired by MthUPO variants represent a step forward toward the use and implementation of UPOs in biocatalytic synthetic pathways of industrial interest. The experimental process involved the reaction of 2-Methoxynaphthalene(cas: 93-04-9).Quality Control of 2-Methoxynaphthalene
The Article related to saccharomyces unspecific peroxygenase protein engineering, Fermentation and Bioindustrial Chemistry: Industrial Chemicals and other aspects.Quality Control of 2-Methoxynaphthalene
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