Tracing the tail of ubiquinone in mitochondrial complex I was written by Angerer, Heike;Nasiri, Hamid R.;Niedergesaess, Vanessa;Kerscher, Stefan;Schwalbe, Harald;Brandt, Ulrich. And the article was included in Biochimica et Biophysica Acta, Bioenergetics in 2012.Application of 605-94-7 This article mentions the following:
Mitochondrial complex I (proton pumping NADH:ubiquinone oxidoreductase) is the largest and most complicated component of the respiratory electron transfer chain. Despite its central role in biol. energy conversion the structure and function of this membrane integral multi-protein complex is still poorly understood. Recent insights into the structure of complex I by X-ray crystallog. have shown that iron-sulfur cluster N2, the immediate electron donor for ubiquinone, resides about 30 Å above the membrane domain and mutagenesis studies suggested that the active site for the hydrophobic substrate is located next to this redox-center. To trace the path for the hydrophobic tail of ubiquinone when it enters the peripheral arm of complex I, an extensive structure/function anal. of complex I from Yarrowia lipolytica was performed monitoring the interaction of site-directed mutants with five ubiquinone derivatives carrying different tails. The catalytic activity of a subset of mutants was strictly dependent on the presence of intact isoprenoid moieties in the tail. Overall a consistent picture emerged suggesting that the tail of ubiquinone enters through a narrow path at the interface between the 49-kDa and PSST subunits. Most notably a set of methionines was identified that seems to form a hydrophobic gate to the active site reminiscent to the M-domains involved in the interaction with hydrophobic targeting sequences with the signal recognition particle of the endoplasmic reticulum. Interestingly, two of the amino acids critical for the interaction with the ubiquinone tail are different in bovine complex I and it could be shown that one of these exchanges is responsible for the lower sensitivity of Y. lipolytica complex I toward the inhibitor rotenone. In the experiment, the researchers used many compounds, for example, 2,3-Dimethoxy-5-methylcyclohexa-2,5-diene-1,4-dione (cas: 605-94-7Application of 605-94-7).
2,3-Dimethoxy-5-methylcyclohexa-2,5-diene-1,4-dione (cas: 605-94-7) belongs to ethers. Relative to alcohols, ethers are generally less dense, are less soluble in water, and have lower boiling points. They are relatively unreactive. Autoxidation is the spontaneous oxidation of a compound in air. In the presence of oxygen, ethers slowly autoxidize to form hydroperoxides and dialkyl peroxides. If concentrated or heated, these peroxides may explode. To prevent such explosions, ethers should be obtained in small quantities, kept in tightly sealed containers, and used promptly.Application of 605-94-7
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem