《Visualization of endogenous β-galactosidase activity in living cells and zebrafish with a turn-on near-infrared fluorescent probe》 was published in Talanta in 2020. These research results belong to Pang, Xiao; Li, Yaqian; Zhou, Zile; Lu, Qiujun; Xie, Ruihua; Wu, Cuiyan; Zhang, Youyu; Li, Haitao. Synthetic Route of C8H8O3 The article mentions the following:
β-Galactosidase (β-gal) is an important biomarker for primary ovarian cancers. Developing noninvasive bioimaging probes for studying the activity of β-gal is highly desirable for cancer diagnosis. Herein, a turn-on near-IR (NIR) fluorescent probe, 2-((6-(((2S, 3R, 4S, 5R, 6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran -2-yl)oxy)-2,3-dihydro-1H-xanthen-4-yl)methylene)malononitrile named DXM-βgal, was rationally designed based on enzymic reaction for the detection of β-gal activity both in vitro and in vivo. Upon incubating with β-gal, DXM-βgal displayed a significant fluorescence enhancement at 640 nm, accompanying by a color change of solution color from red to purple. DXM-βgal exhibited high selectivity and sensitively to β-gal with low limit of detection (2.92 x 10-4 U mL-1). Besides, based on its advantages of long-wavelength emission and excellent biocompatibility, DXM-βgal was successfully applied to imaging β-gal in living cells and zebrafish. Given these prominent properties, we believe that DXM-βgal will be a potential tool for investigating β-gal activity in biomedical research. The results came from multiple reactions, including the reaction of 2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3Synthetic Route of C8H8O3)
2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3) is employed in the synthesis of Schiff base ligand. It is applied as a reactant in the synthesis of LPA1R antagonists used in the inhibition of LPA-induced proliferation and contraction of normal human lung fibroblasts. Also used in the synthesis of tyrosine kinase 6 proteinase inhibitors.Synthetic Route of C8H8O3
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Ether | (C2H5)2O – PubChem