On April 18, 2001, Li, Zuyi; Wu, Zhongliu published a patent.Quality Control of (S)-2-(2-Methoxyphenyl)propanoic acid The title of the patent was Rhodococcus sp. d12 and its culture method and application. And the patent contained the following:
The Rhodococcus sp.D12 (CGMCC Number0497) is prepared by culturing in culture medium at 15-40° for 1-3 d, culturing again in culture liquid at 15-40° for 1-3 d, washing with physiol. saline, and centrifugating or filtering. The culture medium comprises C source 0-20, N source 1-15, 0.5-5 g L-1 phosphate buffer 0-10, NaCl 0-1, microelement 0-5, and agar 10-40 g L-1, and its pH is 3-8. The culture liquid is composed of C source 0-20, N source 1-15, 0.5-5 g L-1 phosphate buffer 0-10, NaCl 0-1, microelement 0-5, and inducer 0.2-10 g L-1, and its pH is 3-8. The C source is selected from glucose, sucrose, fructose, maltose, lactose, xylose, soluble starch, ethanol, Na succinate, mannitol, and soluble dextrin. The N source is selected from yeast extract, beef extract, soya extract, peptone, L-Glu, (NH4)2SO4, NH4Cl, or NH4NO3. The inducer is benzamide, urea, acetonitrile, methacrylonitrile, methacrylamide, isobutyric acid, butyronitrile, acrylamide, formamide, or propionic acid. The microelement is Zn, Mn, Co, Ni, Mg, Fe, Ca, Cu, or B. The Rhodococcus sp. D12 is used as hydrolytic catalyst of nitrile compounds The experimental process involved the reaction of (S)-2-(2-Methoxyphenyl)propanoic acid(cas: 81616-80-0).Quality Control of (S)-2-(2-Methoxyphenyl)propanoic acid
The Article related to rhodococcus nitrile compound hydrolysis acid amide, Fermentation and Bioindustrial Chemistry: Pharmaceuticals (Including Nutrients) and other aspects.Quality Control of (S)-2-(2-Methoxyphenyl)propanoic acid
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