Chatenoud, Lucienne published the artcilePolyclonal and monoclonal antibodies directed against SK & F 94461, a specific H1 histamine receptor ligand, Recommanded Product: N-(4-Methoxybenzyl)pyridin-2-amine, the publication is Molecular Pharmacology (1988), 34(2), 136-44, database is CAplus and MEDLINE.
SK & F 944618 an aminopentyl analog of mepyramine, is a recently described H, receptor antagonist. At variance with the other available H1 receptor ligands, SK & F 94461 offers the possibility of coupling to a protein carrier to render the mol. immunogenic. SK & F 94461 coupled to succinylated bovine serum albumin was used as an immunogen to raise polyclonal antibodies in rabbits and BALB/c mice. In parallel, spleen cells from immunized mice were used to produce hybridomas by somatic cell fusion. Thus, 6 different murine monoclonal antibodies sharing anti-SK & F 94461 specificity were selected for further detailed characterization of their binding properties. Pharmacol. studies of competitive inhibition by using a set of 11 histaminergic agents allowed anal. of the fine specificity of anti-SK & F 94461 antibodies. Both polyclonal and monoclonal anti-SK & F 94461 antibodies showed very high affinity for the immunizing mol. (i.e., Ka values for monoclonal antibodies 8 and 12 were, resp., 3 × 1010 and 1.4 × 1010 M-1). Both types of antibodies bound with high affinity (IC50 ranging from 10-10-10-12 M) to mepyramine, which has a chem. structure closely resembling that of SK & F 94461. Moreover, these antibodies displayed clear-cut stereoselectivity inasmuch as they bound the d-configuration of chlorpheniramine with higher affinity than the l-form. Thus, all 6 monoclonal antibodies showed IC50 values 1-6 log units lower for d– than for l-chlorpheniramine. For some monoclonal antibodies, spectroscopic and fluorescence spectra studies showed that their different binding capacities correlated with their optical properties. Similarly, polyclonal anti-SK & F 94461 antibodies showed a 500-fold lower affinity for l– than for d-chlorpheniramine. All these results indicate that the polyclonal and the majority of monoclonal anti-SK & F 94461 antibodies recognized with high-affinity structural configurations known to be important for the pharmacol. activity of H1 ligands, namely the presence of the dimethylaminoethyl side chain and, with stereochem. selectivity, the d-configuration of chlorpheniramine. These data extend for the 1st time to an H1 histamine receptor ligand results reported in other hormone systems.
Molecular Pharmacology published new progress about 52818-63-0. 52818-63-0 belongs to ethers-buliding-blocks, auxiliary class Pyridine,Amine,Benzene,Ether, name is N-(4-Methoxybenzyl)pyridin-2-amine, and the molecular formula is C13H14N2O, Recommanded Product: N-(4-Methoxybenzyl)pyridin-2-amine.
Referemce:
https://en.wikipedia.org/wiki/Ether,
Ether | (C2H5)2O – PubChem