Visual monitoring of the lysosomal pH changes during autophagy with a red-emission fluorescent probe was written by Wang, Xiaodong;Fan, Li;Wang, Yubin;Zhang, Caihong;Liang, Wenting;Shuang, Shaomin;Dong, Chuan. And the article was included in Journal of Materials Chemistry B: Materials for Biology and Medicine in 2020.Application of 111-77-3 This article mentions the following:
Autophagy plays crucial roles in maintaining normal intracellular homeostasis. Mol. probes capable of monitoring lysosomal pH changes during autophagy are still highly required yet challenging to develop. Here, a lysosome-targeting fluorescent pH probe, RML (I), is presented by introducing a methylcarbitol unit as the lysosome-targeting group to rhodamine B, which is highly sensitive to pH changes. RML exhibits remarkable pH-dependent behavior at 583 nm with a fluorescent enhancement of >148-fold. The pKa value is determined as 4.96, and the linear response with pH changes from 4.50-5.70, which is favorable for lysosomal pH imaging. Also RML diffuses selectively into lysosomes using confocal fluorescence microscopy. Using RML, the authors have successfully visualized autophagy by monitoring the lysosomal pH changes. In the experiment, the researchers used many compounds, for example, 2-(2-Methoxyethoxy)ethanol (cas: 111-77-3Application of 111-77-3).
2-(2-Methoxyethoxy)ethanol (cas: 111-77-3) belongs to ethers. Ether is less polar than esters, alcohols or amines because of the oxygen atom that is unable to participate in hydrogen bonding due to the presence of bulky alkyl groups on both sides of the oxygen atom. But ether is more polar than alkenes. Ethers are good solvents partly because they are not very reactive. Most ethers can be cleaved, however, by hydrobromic acid (HBr) to give alkyl bromides or by hydroiodic acid (HI) to give alkyl iodides.Application of 111-77-3
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem