An assessment of the toxicity of some pesticides and their metabolites affecting a natural aquatic environment using the Microtox system was written by Amoros, I.;Connon, R.;Garelick, H.;Alonso, J. L.;Carrasco, J. M.. And the article was included in Water Science and Technology in 2000.Synthetic Route of C8H9NO3 This article mentions the following:
The conservation and preservation of aquatic ecosystems is of utmost importance due to the high diversity and d. of species and their complex food network. The evaluation of the potential adverse environmental impact caused by pesticides entering water bodies is an important parameter in aquatic toxicity. The toxicity of the insecticide, fenitrothion and two of its metabolites, 3-methyl-4-nitrophenol and 3-methyl-4-nitroanisole, and of the herbicides thiobencarb and molinate, commonly used in rice fields in Valencia near the protected area of lake Albufera, was tested by the Microtox system. The 15 min EC50 values obtained with the marine luminescent bacterium Vibrio fischeri showed that the thiobencarb was the most toxic of the 3 tested pesticides with an EC50 value of 0.03 mg/L. The EC50 values of the first 2 steps of the fenitrothion hydrolysis indicated that while the first metabolite, 3-methyl-4-nitrophenol, was as toxic as its parent compound, a decreased toxicity was observed for the second metabolite, 3-methyl-4-nitroanisole. To analyze the toxic effects of pesticides in complex mixtures the EC50 values of fenitrothion, molinate, and thiobencarb as pure substances and their mixtures were compared. The impact of the pesticides in the natural ecosystem was also studied and a protective effect of lake water was observed In the experiment, the researchers used many compounds, for example, 3-Methyl-4-nitroanisole (cas: 5367-32-8Synthetic Route of C8H9NO3).
3-Methyl-4-nitroanisole (cas: 5367-32-8) belongs to ethers. Ether is less polar than esters, alcohols or amines because of the oxygen atom that is unable to participate in hydrogen bonding due to the presence of bulky alkyl groups on both sides of the oxygen atom. But ether is more polar than alkenes. Electron-deficient reagents are also stabilized by ethers. For example, borane (BH3) is a useful reagent for making alcohols. Pure borane exists as its dimer, diborane (B2H6), a toxic gas that is inconvenient and hazardous to use. Borane forms stable complexes with ethers, however, and it is often supplied and used as its liquid complex with tetrahydrofuran (THF).Synthetic Route of C8H9NO3
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem